Journal: iScience

Article Title: GluK2 Q/R editing regulates kainate receptor signaling and long-term potentiation of AMPA receptors

doi: 10.1016/j.isci.2023.107708

Figure Lengend Snippet:

Article Snippet: Rabbit Polyclonal Anti-GluA3 , Alomone , RRID: AB_2039883.

Techniques: Recombinant, Protein Extraction, Software

Journal: Pharmaceuticals

Article Title: Anti-AMPA Receptor Autoantibodies Reduce Excitatory Currents in Rat Hippocampal Neurons

doi: 10.3390/ph16010077

Figure Lengend Snippet: Rabbit anti-AMPAR GluR3B immunogenicity response. ( A ) ELISA of protein A-purified AMPAR (GluR3) Aabs against the GluR3 immunisation peptide or an irrelevant peptide; n = 3 technical replicates. ( B ) Western blot of mouse whole brain lysate probed with a commercial anti-AMPAR antibody (cAMPAR), anti-AMPAR Aabs, a class-specific negative control rIgG (naïve) or secondary antibody only (negative control). Representative blots from n = 3 technical replicates. ( C ) Immunocytochemical staining of fixed primary cortical neuron on cultures. Cells (DIV8) were stained with anti-AMPAR Aabs (red), anti-βIII tubulin (green), GFAP (white) and nuclei counterstained with DAPI (blue). Examples of labelling of hippocampal neurons with anti-AMPAR Aabs (red) is indicated by the white arrows. Scale bars = 20 μm. Representative images from n = 3 technical replicates.

Article Snippet: Primary and secondary antibodies used were as follows: rabbit anti-AMPAR (1:100; raised against residues 60–73 of rat GluR3 ATD, AGC-010, Alomone Labs, Jerusalem, Israel); rabbit anti-IgG 1 (rIgG, 1:100, 011-000-003, Jackson ImmunoResearch, Cambridge, UK); mouse anti-IgG 2b (1:100, 70–4732, BioLegend, London, UK); mouse anti-βIII-tubulin (mIgG2b, 1:500, 801201, BioLegend, London, UK); mouse anti-glial fibrillary acidic protein (GFAP) (1:400, MAB3402, Millipore); goat anti-rabbit or anti-mouse Alexa Fluor 488/594/647 (all at 1:1000, Life Technologies, Loughborough, UK).

Techniques: Enzyme-linked Immunosorbent Assay, Purification, Western Blot, Negative Control, Staining

Journal: PLoS ONE

Article Title: AMPA Receptor Regulation at the mRNA and Protein Level in Rat Primary Cortical Cultures

doi: 10.1371/journal.pone.0025350

Figure Lengend Snippet: Double labeling of rat cortical neurons at DIV5 and DIV26 (20×) with an antibody against the glial marker GFAP (green) and with antibodies against the four AMPA receptor subunits: (A, B) GluR1; (C, D) GluR2; (E, F) GluR3 and (G, H) GluR4 (red). The images were acquired from rare regions with high density of glial cells to show that the GFAP-positive cells do not express AMPA receptors. Scale bar: 50 µm.

Article Snippet: The membranes were blocked for 60 min with 3% nonfat dry milk or 2% BSA in TBS-T (Tris-buffered saline with 0.1% Tween-20, Sigma-Aldrich) and then incubated overnight at 4°C in the blocking solution with the primary antibodies corresponding to either rabbit polyclonal anti-GluR1 (1∶200, Millipore), rabbit polyclonal anti-GluR2 (1∶2500, Millipore), rabbit polyclonal anti-GluR3 (1∶500, Alomone Labs Ltd) or rabbit polyclonal anti-GluR4 (1∶100, Millipore) with mouse monoclonal anti-GAPDH (1∶2500, Sigma-Aldrich) as a loading control.

Techniques: Labeling, Marker